Figure 4.

MAGEL2 WT and p.Gln666Profs^∗47 co-localize with EEA1, RAB11, SMN and FMRP in mouse Neuro-2a cells

Mouse Neuro-2a cells were transiently transfected with WT mCherry-MAGEL2 or mCherry-p.Gln666Profs^∗47 (magenta) fusion proteins and subcellular localization was analyzed employing confocal immunofluorescence microscopy 24 h after transfection. Nuclei were counterstained with DAPI (blue). The scale bar is 10 μm. SMN (A), FMRP (B), endogenous early endosomes (C), and recycling endosomes (D) were visualized with anti-SMN, anti-FMRP, anti-EEA1, and anti-RAB11A antibodies (green), respectively. A white signal in the overlap images indicated signal overlap or co-localization. At least 3 transfected cells were imaged in three independent replicates and representative cells are depicted.