Figure 5.
SNORD116 does not directly interact with WT MAGEL2 or p.Gln666Profs∗47
RNA-coIP was performed with biotinylated SNORD116, SNORD100, or a SCR control with HEK293T IP lysates. Analyses were carried out with proteins identified in at least 2 of 3 replicates per condition.
(A) Venn diagrams showing overlapping proteins identified via RNA-coIP. The 97, 194, and 144 proteins found in RNA-coIPs for SNORD116 and SNORD100 (underlined), SNORD100 with overlap (dashed circles), and SNORD116 with overlap (dashed circles) were employed for the analysis in (B).
(B) GO term enrichment analysis employing enrichR for Biological Processes (2023) for proteins identified in SNORD116 and SNORD100, but not in the SCR control. Top 5 hits by Combined Score60 are shown in shades of blue. Gray circles indicate that the respective GO term is among top hits in another condition. Only GO terms with an adjusted p value < 0.05 were included.
(C) Venn diagram displaying the overlap between unique SNORD116 interacting proteins (A) and those identified in 2 of 3 replicates of the BioID or GFP-CoIP performed with WT MAGEL2 or p.Gln666Profs∗47.
(D) Table showing overlapping proteins from (C).