Fig. 1.

Low NRIP expression in the spinal cord and skeletal muscles of SOD1 G93A mice. A NRIP expression in the spinal cord at age of 56 days WT and SOD1 G93A mice. Mice were euthanized, and transcardial perfusion with PBS was performed to remove blood from tissues. Total proteins from L3-L5 spinal cord were subjected to Western blot (WB) analysis for NRIP expression, with GAPDH serving as the loading control. The asterisk indicates the representative band for NRIP expression. The right panel represents the quantification of NRIP expression conducted through densitometry analysis. B NRIP expression in the gastrocnemius (GAS) muscles. The asterisk indicates the representative band for NRIP expression. The right panel illustrates the quantification. C NRIP expression in the tibialis anterior (TA) muscles. The asterisk indicates the representative band for NRIP expression. The right panel shows the quantification. WT, N = 3 mice; SOD1 G93A, N = 5 mice. D NRIP expression in the lumbar segment of the spinal cord at the age of 56 days WT and SOD1 G93A mice. The 30 μm-thick sections were co-stained with anti-ChAT (green) and anti-NRIP (red) antibodies to detect NRIP expression in spinal cord motor neurons. DAPI was used as the nuclear counterstain. Arrows indicate high NRIP expression in ChAT-positive neurons, while arrowheads indicate low NRIP expression in ChAT-positive neurons. Scale bar: 100 μm. Right panel: Quantification of the immunofluorescence intensity of NRIP in ChAT-positive neurons from WT mice (N = 3) and SOD1 G93A mice (N = 3). Data are presented as mean ± SEM. Statistical analysis was conducted using the Student t-test. *P < 0.05 and ***P < 0.001