TABLE 1.
Bacterial strains and plasmids used in this study
| Strains/plasmids | Relevant characteristic | Sources |
|---|---|---|
| Strains | ||
| E. coli | ||
| Top10 | F- mcrAΔ(mrr-hsdRMS-mcrBC) φ80lacZ ΔM15 ΔlacX74 nupG araD139Δ(ara-leu)7697 galE15 galK16 rpsL(StrR) endA1 λ- | Takara Bio Inc |
| JM109 | F- endA1 glnV44 thi-1 gyrA96 recA1 mcrB+Δ(lac-proAB) e14- (traD36 proAB+ lacIq lacZΔM15) hsdR17(rK- rK+) | Takara Bio Inc |
| WM3064 | ThrB1004 pro thi rpsL hsdS lacZΔM15 RP4-1360 Δ(araBAD)567ΔdapA1341::(erm pir) | Gift of Xiaoxue Wang from the South China Sea Institute, Chinese Academy of Sciences |
| B. choshinensis | ||
| B. choshinensis | Wild-type strain | Takara Bio Inc |
| B. choshinensis-PhSULF1 | B. choshinensis with the plasmid expressing PhSULF1 | This work |
| B. choshinensis-PhSULF2 | B. choshinensis with the plasmid expressing PhSULF2 | This work |
| P. haloplanktis | ||
| P. haloplanktis LL1 | Wild-type strain | MCCC |
| ∆SULF1 | P. haloplanktis LL1 with deleted PhSULF1 | This work |
| ∆SULF2 | P. haloplanktis LL1 with deleted PhSULF2 | This work |
| Plasmids | ||
| pNCMO2 | Expression vector | Takara Bio Inc |
| pNCMO2-PhSULF1 | pNCMO2 derivative for expressing PhSULF1 | This work |
| pNCMO2-PhSULF2 | pNCMO2 derivative for expressing PhSULF2 | This work |
| pK18mobsacB-Cm | Suicide vector | Gift of Xiaoxue Wang from the South China Sea Institute, Chinese Academy of Sciences |
| pK18mobsacB-Cm-SULF1 | pK18mobsacB-Cm derivative for deleting PhSULF1 | This work |
| pK18mobsacB-Cm-SULF2 | pK18mobsacB-Cm derivative for deleting PhSULF2 | This work |