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Summary of targeting paaA with RhaCAST system in B. multivorans ATCC 17616
| PAM | Distance between ATG and downstream PAM (bp) | Conjugation efficiency (conjugant/recipient) |
Colonies on target (mutant with expected phenotype/isolate screened) | Percent (%) on target |
|---|---|---|---|---|
| CGTC | 435 | 7.22 × 10−9 | 12/13 | 92 |
| GGTT | 80 | 4.33 × 10−9 | 1/9 | 11 |
| GGTC | 193 | 1.60 × 10−8 | 30/31 | 97 |
