. 2024 Jun 13;90(7):e00699-24. doi: 10.1128/aem.00699-24

TABLE 3.

Long-read sequencing summary of mutants created using RhaCAST system in B. cenocepacia K56-2 and B. multivorans ATCC17616

Mutants	crRNA’s primer number	Summary	Distance of cargo integration (bp)^a
K56-2 paaA::CAST-Tp	3390	On-target, integration of cargo only	63
K56-2 paaA::CAST-Tp	3422	On-target, integration of cargo only	61
K56-2 paaA::CAST-Tp	3423	On-target, integration of cargo only	63
K56-2 fliF::CAST-Tp	3463	On-target, integration of cargo only	67
K56-2 fliF::CAST-Tp	3466	On-target, integration of cargo only	64
K56-2 phbC::CAST-Tp	3469	On-target, integration of cargo only	64
K56-2 phbC::CAST-Tp	3472	On-target, integration of cargo only	64
K56-2::CAST-fugfp-Tp	3475	On-target, integration of cargo only	63
K56-2::CAST-fugfp-Tp	3478	On-target, integration of cargo only	64
K56-2::CAST-fugfp fliF::CAST-Tp	3475	On-target, integration of cargo only	60
K56-2::CAST-fugfp fliF::CAST-Tp	3475	On-target, whole-plasmid integration^b	63
ATCC 17616 paaA::CAST-Tp	3390	On-target, integration of cargo only	59
ATCC 17616 paaA::CAST-Tp	3422	On-target, whole-plasmid integration^b	65
ATCC 17616 paaA::CAST-Tp	3424	On-target, integration of cargo only	59
ATCC 17616 fliF::CAST-Tp	3463	On-target, whole-plasmid integration^b	60

^{^a}

From PAM to LE.

^{^b}

Co-integration of the cargo DNA and the whole plasmid.