Fig 1.

Specific activities of AA5_2 members on representative compounds. Individual values are presented in Table S2. Specific activity was determined using the HRP-ABTS-coupled assay with 50 mM sodium phosphate buffer, pH 7.0, at room temperature, except for NexGalOx (50 mM sodium acetate buffer, pH 5.0). Measurements were performed in triplicate with 300 mM carbohydrates (galactose, lactose, melibiose, and raffinose), 300 mM glycerol, 10 mM aromatic alcohols [benzyl alcohol and 5-hydroxymethylfurfural (HMF)], and 10 mM aldehydes (methyl glyoxal and glyoxal). Nha, Nectria haematococca; Efe, Epichloe festucae; Nex, Niesslia exilis; Ast, Acremonium strictum; Bsp, Bisporella sp.; and Xpa, Xanthoria parietina.