TABLE 2.
Comparison of amino acid substitutions in key active site positions of several characterized AA5_2 members and the archetype FgrGalOxa
| Enzyme | Organism | Active site amino acids | GenBank/JGI accession | Reference | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Radical stabilization | Active site shape/substrate recognition | |||||||||
| FgrGalOx | Fusarium graminearum | W290 | F194 | Q326 | Y329 | R330 | Q406 | F464 | AAO95371 | (16, 19) |
| FsaGalOx | Fusarium sambucinum | W | F | Q | Y | R | Q | F | AIR07394 | (50) |
| FauGalOx | Fusarium austro- americanum | W | F | Q | Y | R | Q | F | AAA16228 | (45) |
| MreGalOx | Mytilinidion resinicola | W | F | Q | F | R | Q | F | XP_033570565 | (40) |
| ExeGalOx | Exophiala xenobiotica | W | F | Q | F | R | Q | F | KIW55415 | (40) |
| FoxGalOxB | Fusarium oxysporum | W | M | Q | F | R | Q | F | FOTG_04629 | (40) |
| PfeGalOx | Penicillium fellutanum | W | Y | Q | Y | R | Q | F | jgi|Penfe1|382062 | (40) |
| Xpa GalOx | Xanthoria parietina | W | F | R | Y | R | Q | F | jgi|Xanpa2|1578647 | This work |
| Bsp GalOx | Bisporella spp. | W | Y | Q | F | R | Q | F | jgi|Bissp1|110239 | This work |
| Efe GalOx | Epichloe festucae | W | F | R | Y | R | D | F | ACN30267 | This work |
| Nex GalOx | Niesslia exilis | W | F | Q | F | R | Q | F | jgi|Nieex1|192818 | This work |
| Nha GalOx | Nectria haematococca | W | F | E | Y | R | R | F | XP_003039318 | This work |
| FveGalOx | Fusarium verticillioides | W | F | E | Y | K | Q | F | ADG08188 | (45) |
| FsuGalOx | Fusarium subglutinans | W | F | E | Y | K | Q | F | ADG08187 | (46) |
| Pru AlcOx/GalOx | Penicillium rubens | W | Y | D | Y | R | E | F | CAP96757 | (40, 43) |
| CgrRafOx | Colletotrichum graminicola | Y | F | A | W | R | S | F | EFQ36699 | (42) |
| CgrAlcOx | Colletotrichum graminicola | F | W | G | F | M | T | F | EFQ30446 | (32) |
| CglAlcOx | Colletotrichum gloeosporioides | F | W | L | F | M | T | F | jgi|Gloci1|1901294 | (32, 40) |
| PorAlcOx | Pyricularia oryzae | F | F | G | L | Y | T | F | XP_003719369 | (40) |
| AsyAlcOx | Aspergillus sydowii | F | Y | H | D | R | V | F | XP_040706357 | (40) |
| AflAlcOx | Aspergillus flavus | W | Y | L | Y | H | E | F | KAF7627372 | (40) |
| FoxAlcOx | Fusarium oxysporum | W | F | D | S | K | A | F | EXL65576 | (40) |
| FoxAAO | Fusarium oxysporum | W | F | E | Y | K | Q | F | XP_018246910 | (41) |
| FgrAAO | Fusarium graminearum | W | F | E | Y | K | Q | F | XP_011322138 | (41) |
| CgrAAO | Colletotrichum graminicola | Y | F | E | W | R | T | F | EFQ27661 | (33) |
| Ast AAO | Acremonium strictum | W | F | E | S | K | R | F | jgi|Acrst|1377707 | This work |
a
Conserved residues in relation to FgrGalOx are indicated in bold font and non-conserved residues are indicated in italic font, based on primary and tertiary structural alignments. AA5_2 members characterized in this present study are indicated in underlined, bold font.