TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Description | Source |
|---|---|---|
| E. coli strains | ||
| DH5α | DNA propagation | Invitrogen |
| S17.1 | Conjugation of plasmids into RHA1 | (36) |
| Rhodococcus strains | ||
| R. jostii RHA1 | Wild type | (26) |
| R. jostii RHA1-EG | EG-adapted RHA1 | This study |
| ΔmadA | RHA1 deletion mutant of madA | This study |
| ΔmftC | RHA1 deletion mutant of mftC | This study |
| Plasmids | ||
| pK18mobsacB | sacB, oriT, aphII | (37) |
| pTip-QC2 | Expression in RHA1, PtipA, Chlr, repAB | (38) |
| pK18ΔmadA | pK18mobsacB-derived construct to delete madA | This study |
| pK18ΔmftC | pK18mobsacB-derived construct to delete mftC | This study |
| pTip-madA | pTip-QC2 harboring madA | This study |
| pTip-mftC | pTip-QC2 harboring mftC | This study |
| pTip-aldA2 | pTip-QC2 harboring aldA2 | This study |