Fig 2.
The lacI-Ptrc inducible system for R. nubinhibens. (A) Schematic representation of the pmCherry. The lacI gene is driven by a constitutive promoter, and the mCherry fluorescence gene is driven by the Ptrc inducible promoter. (B) Fluorescence of strains with pBBR1MCS-5, as a control, and pmCherry under different IPTG concentrations. The value of fluorescence intensity (A.U., arbitrary unit) was normalized by optical density at 600 nm with a final OD600 of 0.01. Experiments were carried out in quintuplicate, and the error bars represented the standard deviations of the means of five biological replicates. The differences were statistically evaluated by the t-test. (C) Count of mCherry fluorescent cells. (D) Distribution of mCherry fluorescent cells in the population. For each sample, 10,000 events were analyzed.