Figure 3. Peripheral effects of pressure-enabled drug delivery of nelitolimod in combination with Sys or SQ α-programmed cell death-1. The serum collected on D3 was analyzed for (A) IFN-γ and (B) CXCL10 (IP-10) were measured by Luminex and reported as fold change compared with Veh. Liver function tests were performed to monitor the level of (C) AST, (D) total bilirubin, (E) total protein, and (F) BUN. Normal ranges for C57/Bl6 AST: 46–221 U/L, bilirubin: 0.5–1.1 mg/dL, total protein: 4.6–7.3 g/dL and BUN: 2–71 mg/dL. Animal data were presented as mean±SEM and n was mentioned in the individual graph. One-way analysis of variance and multiple t-test was performed to determine statistical differences among multiple groups. CXCL10, C-X-C motif chemokine ligand 10; IP-10, Interferon-gamma-induced protein 10; AST, aspartate aminotransferase; IFN, interferon; SQ, subcutaneous; Sys, systemic; Veh, vehicle.