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. 2024 Jun 12;300(7):107463. doi: 10.1016/j.jbc.2024.107463

Figure 5.

Figure 5

Structural determinants of USP36-cIAP1 and USP36-survivin interactions. A, schematic representation of the USP36 and its deletion mutants used in this work. B, HA-survivin was expressed along with full-length USP36 (FL) or each of the indicated Myc-USP36 mutants. Cell lysates were immunoprecipitated with anti-Myc beads and blotted with anti-Myc and anti-HA antibodies. C, HA-survivin was expressed with 1 to 420 or 421 to 800 Myc-USP36 mutant. Cell lysates were immunoprecipitated with anti-HA beads and blotted with anti-HA and anti-Myc antibodies. D, Myc-cIAP1 was expressed along with each of the indicated HA-USP36 mutants. Cell lysates were immunoprecipitated with anti-Myc beads and blotted with anti-Myc and anti-HA antibodies. E, HEK-293 cells were transfected with survivin alone or together with Myc-tagged full-length USP36 or USP36 (1–420aa). Proteins were extracted and blotted with the indicated antibodies. F, HEK-293 cells were transfected with survivin alone or together with Myc-tagged full-length USP36 or USP36 (C131A). Proteins were extracted and blotted with the indicated antibodies. G, HEK-293 cells were transfected with cIAP1 alone or together with HA-tagged full-length USP36 or USP36 (C131A). Proteins were extracted and blotted with the indicated antibodies. HA, hemagglutinin; HEK, human embryonic kidney; IAP, inhibitor of apoptosis protein; USP36, ubiquitin-specific protease 36.