Fig. 6. Sustained macrophage ablation protected against cisplatin-induced OHC death and resulted in reduced cisplatin accumulation in the cochlea (experiment 2).

(A and B) Cochlear wholemounts were stained for Myosin 7a (cyan) to visualize hair cells. (A) Representative images and (B) quantitative analysis of Myosin 7a–positive hair cells. Scale bar, 100 μm. (B) Data are shown as means ± SEM, n = 6 cochleae (3 females and 3 males) per experimental group (total 24 cochleae; 12 females and 12 males). Statistical analysis was performed using two-way ANOVA with Tukey’s multiple comparisons test (main column effect). (C) Platinum levels were measured using ICP-MS in microdissected inner ear tissues. Data are shown as means ± SD, n = 4 to 6 cochleae (2 to 3 females and 2 to 3 males) per experimental group (total 22 cochleae; 12 females and 10 males). Statistical analysis was performed using one-way ANOVA with Tukey’s multiple comparisons test.