Table 3. Results of the validation process for the HPLC method of measuring the levels of lincomycin in the plasma of broiler chickens.

Method validation parameters	Method validation results	Information
Linearity	The r2 values resulting from spiking lincomycin with concentrations of 50 μg/ml, 25 μg/ml, and 10 μg/ml in broiler chicken plasma are: r2 = 0.9983	Good
Accuracy	The % recovery value is between 90% and 110%	Good
Precision	% coefficient of variation (CV) value ≤ 15%	Good
Specificity	No peak area appears in the chromatogram results of the blank sample. The lincomycin peak area appeared in the chromatogram results of the spiking sample at the 10th minute.	Good
LOD	Limit of quantification = 1.12 μg/ml	Good
LOQ	Limit of Quantification= 4,86 μg/ml	Good