Fig. 3.

Heptane alone does not induce inositol acylation. A: HPTLC analysis of PIMs extracted into CMW from live and heat-killed cells. Cells were either untreated (UT, lane 1), treated with RMS (lanes 2&3), or treated with heptane (lanes 4&5) before CMW extraction. PIMs were analyzed as in Fig. 2A (Rf = 0.28–0.65). B: Lipids were extracted into RMS with varying concentrations of sodium docusate, and the remaining lipids were extracted into CMW. The HPTLC analysis (as in Fig. 2A) of the CMW extracts is shown (Rf = 0.24–0.63).