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Effect of medium components on PIM inositol acylation. Cells were grown to log or early stationary phase in Middlebrook 7H9 supplemented with ADC or DC and Tween-80. PIMs were extracted into CMW after the RMS treatment or directly into CMW. PIMs were analyzed as in Fig. 2A (Rf = 0.26–0.66). DC, dextrose-sodium chloride.
