FIG. 1.

Phosphorylation mutants of VP22. At the top is the endolysine C cleavage map of the 301-amino-acid VP22 open reading frame (ORF), with consensus serine CKII sites denoted. Each of the cleavage products is labelled a to h. The regions of the ORF expressed from plasmids pUL49ep (Wt) and Δ267 and the serine (S)-to-alanine (A) substitutions present in plasmids pGE160, pGE161, pGE167, and pGE168 are shown. These substitutions were introduced by PCR mutagenesis into the parental plasmid pUL49ep.