Table 1.

Overview of the patient cohort, including epidemiology (age, sex, tumor location, type of lesion, type of non-surgical pre-treatment, routine neuropathological results (microarray methylation class) and nanopore sequencing results (nanopore methylation class and methylation class family with scores, tSNE concordance)

Patient ID	Age	Sex	Tumor location	Reference diagnosis	Type of lesion	Type of non-surgical pre-treatment	Microarray methylation class (MC)	Nanopore methylation class (MC)	Score MC	Nanopore methylation class family (MCF)	Score MCF	tSNE concordance
DX-BLN-025	8	F	Parieto-occipital right	CNS neuroblastoma	Primary	None	CNS neuroblastoma with FOXR2 activation	CNS neuroblastoma with FOXR2 activation	0.68	CNS neuroblastoma with FOXR2 activation	0.68	+
DX-BLN-027	17	F	Right trigeminal nerve	Schwannoma	Primary	None	schwannoma	schwannoma	0.85	schwannoma	0.85	+
DX-BLN-028	9	F	Right cerebellar hemisphere	Pilocytic astrocytoma	Primary	None	low grade glioma, subclass posterior fossa pilocytic astrocytoma	low grade glioma, subclass posterior fossa pilocytic astrocytoma	0.44	pilocytic astrocytoma	0.58	+
DX-BLN-029	1	F	Fronto-temporo-parietal right	Ependymoma	Secondary	Cyclophosphamide/vincristine/methotrexate/carboplatin/etoposide	ependymoma, YAP fusion	ependymoma, YAP fusion	0.07	glioblastoma, IDH wildtype	0.10	+
DX-BLN-037	5	F	Right cerebellar hemisphere	Pilocytic astrocytoma	Secondary	Vincristine/carboplatin	low grade glioma, subclass posterior fossa pilocytic astrocytoma	low grade glioma, subclass posterior fossa pilocytic astrocytoma	0.33	pilocytic astrocytoma	0.54	+
DX-BLN-042	9	F	Left cerebellar hemisphere	Pilocytic astrocytoma	Primary	None	low grade glioma, subclass posterior fossa pilocytic astrocytoma	low grade glioma, subclass posterior fossa pilocytic astrocytoma	0.46	pilocytic astrocytoma	0.56	+
DX-BLN-043	10	M	Left frontal	Ependymoma, RELA fusion-positive	Primary	None	ependymoma, RELA fusion	ependymoma, RELA fusion	0.43	ependymoma, RELA fusion	0.43	+
DX-BLN-044	9	F	Left temporo-parieto-thalamic	High grade glioma, H3 K27M mutated	Secondary	None	Diffuse midline glioma, K27-mutant	Diffuse midline glioma, K27-mutant	0.66	Diffuse midline glioma, K27-mutant	0.66	+
DX-BLN-047	8	F	Left mesencephalic	Pilocytic astrocytoma	Secondary	Vincristine/carboplatin	low grade glioma, subclass posterior fossa pilocytic astrocytoma	low grade glioma, subclass posterior fossa pilocytic astrocytoma	0.34	pilocytic astrocytoma	0.50	+
DX-BLN-048	3	M	IV. Ventricle	Anaplastic Ependymoma	Primary	None	ependymoma, posterior fossa group A	ependymoma, posterior fossa group A	0.75	ependymoma, posterior fossa group A	0.75	+
DX-BLN-049	3	F	Thoracic spine	Pilocytic astrocytoma	Primary	None	low grade glioma, subclass posterior fossa pilocytic astrocytoma	low grade glioma, subclass midline pilocytic astrocytoma	0.07	pilocytic astrocytoma	0.12	-
DX-BLN-050	15	F	Right lateral ventricle	Atypical central neurocytoma	Primary	None	central neurocytoma	central neurocytoma	0.72	central neurocytoma	0.72	+
DX-BLN-054	16	F	Left frontal	Diffuse astrocytoma, IDH-mutant	Primary	None	IDH glioma, subclass astrocytoma	control tissue, hemispheric cortex	0.08	pilocytic astrocytoma	0.17	-
DX-BLN-064	6	F	Foramen Monroi right	Subependymal giant cell astrocytoma	Primary	None	N/A	low grade glioma, subependymal giant cell astrocytoma	0.15	low grade glioma, subependymal giant cell astrocytoma	0.15	-
DX-BLN-065	6	M	Right mesencephalic	Pilocytic astrocytoma	Secondary	Vincristine/carboplatin	low grade glioma, subclass posterior fossa pilocytic astrocytoma	low grade glioma, subclass posterior fossa pilocytic astrocytoma	0.24	pilocytic astrocytoma	0.47	+
DX-BLN-071	7	F	Optic pathway	Pilocytic astrocytoma	Secondary	Vincristine/carboplatin, vinblastine, MEK inhibitor	N/A	low grade glioma, subclass midline pilocytic astrocytoma	0.37	pilocytic astrocytoma	0.48	+
DX-BLN-073	7	M	Sella turcica	Craniopharyngioma	Primary	None	craniopharyngioma, adamantinomatous	craniopharyngioma, adamantinomatous	0.56	craniopharyngioma, adamantinomatous	0.56	+
DX-BLN-074	6	M	Right cerebellar hemisphere	Pilocytic astrocytoma	Primary	None	low grade glioma, subclass posterior fossa pilocytic astrocytoma	low grade glioma, subclass posterior fossa pilocytic astrocytoma	0.58	pilocytic astrocytoma	0.69	+
DX-BLN-075	9	M	Right cerebellar hemisphere	Pilocytic astrocytoma	Secondary	None	low grade glioma, subclass posterior fossa pilocytic astrocytoma	low grade glioma, subclass posterior fossa pilocytic astrocytoma	0.32	pilocytic astrocytoma	0.40	+