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. 2024 Jul 24;15:6242. doi: 10.1038/s41467-024-50639-9

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — A Maps of the two plasmid backbones that housed most of the 301 BioBricks that were tested and the five BFP controls that were included in every assay. The prefix (pre) and suffix (suf) multiple cloning sites used in BioBrick assembly are shown. B Burden of each BioBrick tested. Burden is the percentage reduction in the growth rate of E. coli cells transformed with a BioBrick plasmid. Gray points are individual measurements. Bars are the means for all measurements of a BioBrick. For BioBricks with orange bars, the measured burden was significantly greater than zero (adjusted p < 0.05, one-tailed t-tests with Benjamini–Hochberg correction for multiple testing). Supplementary Data 2 contains the results of all microplate assays. Supplementary Data 3 has calculated statistics. Source data are provided with this paper.