Fig. 1.

ISO inhibited HSFBs proliferation. A. Chemical structure of ISO. B. Calcein-AM/PI double staining for cell viability of HSFBs after incubation with ISO (0, 25, 50, 100 μM) for 24, 48, and 72 h. Living cells are labeled by green fluorescence, while dead cells are labeled by red fluorescence. The quantification of the percentage of dead cells. C. EdU staining of HSFBs after treatment with ISO (0, 25, 50, 100 μM) for 48 h. Left panel: the representative images of Edu staining. Right panel: quantification of the percentage of EdU-positive cells. *p < 0.05, **p < 0.01, ***p < 0.001.