Fig. 4.

ISO inhibited the gel contraction, myofibroblast activation, and collagen expression. A. Images and quantitative results of collagen gel contraction assay. Dotted lines indicated the gel areas. n = 3. B. Immunofluorescence staining of α-SMA in HSFBs and TGF-β1-stimulated NFBs. C. Western blotting results of α-SMA in HSFBs and TGF-β1-stimulated NFBs after treatment with ISO (0, 25, 50, 100 μM) for 48 h and the quantification of the protein levels normalized to GAPDH. D. Western blotting results of COL Ⅰ and COL Ⅲ in HSFBs and the quantification of COL Ⅰ and COL Ⅲ normalized to GAPDH. All assays were conducted with HSFBs after treatment with ISO of different concentrations (0, 25, 50, 100 μM) for 48 h. *p < 0.05, **p < 0.01, ***p < 0.001.