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. 2024 Jun 29;10(13):e33802. doi: 10.1016/j.heliyon.2024.e33802

Fig. 7.

Fig. 7

ISO attenuated rabbit ear hypertrophic scar formation. A. The gross appearances of scars in the control group (treatment with DMSO) and the ISO group (treatment with 100 μM of ISO). B. H&E staining images and area quantification of cross-sections of hypertrophic scars from the control and ISO groups. Dotted lines indicate the scar areas. C. Masson Trichrome staining images and the quantification of SEI. The dotted lines “D” indicates the thickness of rabbit hypertrophic scars. The dotted lines “d” indicates the thickness of the adjacent normal skin. SEI is defined by the D/d ratio. D. Immunohistochemistry images of α-SMA from the DMSO and ISO groups. The quantification of the percentage of α-SMA-positive cells. E. The mRNA expression of TGF-β1, CREB3L1, COL Ⅰ, COL Ⅲ, and α-SMA in DMSO- and ISO-treated scar tissues detected by qRT-PCR and the quantification of the mRNA levels normalized to GAPDH. *p < 0.05, **p < 0.01, ***p < 0.001.