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E2 does not bind COS cell-expressed CD81. KM3-CD81 and COS cells were monitored for CD81 expression with MAbs JS81 (A) and 5A6 (B). Soluble E2661 (open graphs) and mock antigen (shaded graphs) were monitored for binding to KM3-CD81 and COS cells (C) with MAb 11/4b. E2 and mock antigens were also tested for the ability to bind KM3 cells, resulting in a background median FI of 3.0.
