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Recombinant CD81 interaction with E2. A subsaturating amount of soluble E2661 was incubated with increasing concentrations of GST-EC2, EC1.EC2-Fc, or EC2-Fc for 1 h at room temperature and evaluated for its ability to bind RBL-CD81 cells; median FIs are shown. E2661 and mock antigens failed to bind to the parental RBL cells in the same experiment, giving a background median FI of 5.4.
