FIGURE 5.

(A) Cell viability determined by Alamar blue staining of hMSCs grown on 3D model with scaffold loaded with 10 mg of vancomycin hydrochloride or 5 mg of gentamicin sulphate or unloaded scaffold. (B–D) Live and dead cell staining conducted on the scaffolds inserted between the two scaffolds previously seeded with hMSCs; (B) scaffold loaded with 10 mg of vancomycin hydrochloride, (C) scaffold loaded with 5 mg of gentamicin sulfate and (D) unloaded scaffold after 14 days of culture. Viable cells stain green while dead cells stain red: images show that cells grew regularly into the scaffolds as evidenced by the green-fluorescent dye in the absence of the red fluorescence dye (10 × magnification: bar = 100 μm).