TABLE 1.
Primers used for sequencing of RW 98
| Protein | Designation (position)a | Sequence | Product size (bp) | Annealing conditions |
|---|---|---|---|---|
| p40 | p40-11 (11) | 5′-CAA CAA ACC ACT CAT CAT TC-3′ | 401 | 50°C, 90 s |
| p40-412 (412) | 5′-CCG TAA AAC TTC GCA GTC-3′ | |||
| p40 | p40-259 (259) | 5′-TTC ACA CAG TAA CGC CTA GC-3′ | 917 | 58°C, 60 s |
| p40-2 (1176) | 5′-TCA TTG TTT TTT AGT TTA GAC CAG T-3′ | |||
| p10 | p40-3 (1108) | 5′-GAG CTC TCA GGT GAG ATC TCT-3′ | 383 | 50°C, 90 s |
| p24 rev (1491) | 5′-GCT CAT CAT TCG ACA GCT GC-3′ | |||
| p24 | p24-A (1387) | 5′-TGA CCC AAC CAG TAG ACC A-3′ | 478 | 58°C, 60 s |
| p24-B (1865) | 5′-GTC CCA TTC ATC CGT TGT C-3′ | |||
| gp18 | gp18-1 (1882) | 5′-ATC GAA TCA CCA TGA ATT CAA AAC-3′ | 558 | 60°C, 90 s |
| gp18-2 (2340) | 5′-AGT GGA GTC AGT ATT GCA GCT AA-3′ | |||
| gp94 | p57-1 (2224) | 5′-AGC ACG CAA TCA ATG CAG CCT TC-3′ | 623 | 60°C, 90 s |
| p57-3 (2847) | 5′-GGA TGA GTT GCA GAG TAT GGT C-3′ | |||
| gp94 | p57-4 (2780) | 5′-CCC CGT ATA TCC TAC AGT CAG AAG-3′ | 980 | 60°C, 90 s |
| p57-2 (3760) | 5′-TGG TCG GTA CGG TTT ATT CCT GC-3′ |
a
The indicated nucleotide positions refers to the sequence of strain V (accession no. U04608).