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Membrane modification in E. coli by JEV NS4B. (A) Hydrophobicity plot of NS4B by the Kyte-Doolittle method. (B) Schematic diagram of NS4B regions expressed by plasmid constructs, shown in nucleotide numbers (amino acid numbers are in parentheses). (C, D, and E) Membrane permeability assay (described in the legend to Fig. 2).
