FIG. 7.

Effects of expression of JEV NS proteins on reporter activity in BHK-21 cells. BHK-21 cells were cotransfected with a reporter gene and plasmids expressing various JEV NS proteins. The reporters include a luciferase gene (A and B) and a β-galactosidase gene (C and D). At 24 h (A and B) or 48 h (C and D) posttransfection, the cells were lysed and assayed for their luciferase or β-galactosidase activities. 2A-R and 4A-R in panels B and D are the constructs with inserts in opposite orientation.