FIG. 1.

(A) Diagram of the RSV genome containing the selectable marker neo in the place of the src gene. LTR, long terminal repeat; Gag, viral structural protein; Pol, polymerase protein containing reverse transcriptase and integrase; Env, envelope protein. (B) Details of the Gag polyprotein. Amino acid numbering is from the amino terminus of Gag. (C) Gag constructs used to determine domains required for the specific viral RNA packaging into virions. End parts of the deletions are indicated by breaks flanked by amino acid numbers. PR* is a Gag polyprotein carrying an active-site mutation, D37N (indicated by asterisks), in the protease domain, which abolishes protease activity. In myr-ΔMA, the sequence encoded by the first 10 codons of p60^v-src comprising a myristylation signal (64) were added to the N terminus of a Gag polyprotein lacking MA. Amino acids comprising the second Cys-His box in the NC domain are indicated by dots. The locations of the mutated residues in NC-SKL and RTL are indicated below the amino acid sequence, in italics.