FIG. 8.

Effects of Mψ mutations on RNA packaging. (A) RIPA to determine the number of viral particles released from cultures transfected with indicated plasmids. (B) RPA to measure the amount of neo-specific RNA packaged in virions. (C) Comparison of the β-gal activity measured in the yeast three-hybrid system and the packaging efficiency determined in vivo. The packaging efficiency was calculated as the ratio of the relative amount of neo-specific RNA packaged in particles, as measured by RPA (Fig. 8B), to the relative number of particles, as measured by RIPA (Fig. 8A). Both packaging efficiencies and β-gal activities were normalized to PR*. Each experiment was done three or four times, and the bars show the standard deviations.