FIG. 5.

Summary of nuclease digestion data for I12Lss mutant RSV RNA fragments. Secondary-structure diagrams for I12Lss sequences are depicted as described in Materials and Methods. Mutant nucleotides are denoted by italicized lettering. Other notations are as described in the legend to Fig. 3. (A) Digestion of uncomplexed I12Lss RNA fragments. Description of cleavages is as in the legend to Fig. 3A. , the exact cleavage site was unclear, although the data did indicate that cleavage was somewhere within the U5-IR stem. (B) Digestion of I12Lss RNA fragments complexed with tRNA^Trp, where the tRNA^Trp sequences are shown in bold capital letters. Description of cleavages is as in the legend to Fig. 3B, except for V₁ cleavages, where there was no dominant cleavage with 0.0001 U of V₁ per μl; therefore, there is no v v v designation. (C) Changes in the digestion pattern when I12Lss RNA fragments were complexed with tRNA^Trp. Description of changes is as in the legend to Fig. 3C.