Fig 1.
Schematic representation of BKD-operon in L. monocytogenes F2365 strain. (A) BKD-operon consists of six genes, including ptb, buk, and four genes encoding BKD. Downstream of the BKD-operon is sbrA and prli42. The region upstream of the buk contains two putative σA- and σB-dependent promoters. The putative PrfA-binding site is located within buk gene. Two transposon insertional mutants cld-1 and cld-2 constructed by disrupting bkdB and lpd genes are marked with a triangle (14). Putative σA- and σB-dependent promoters in the BKD-operon are marked with arrows. (B) The putative −35 and −10 boxes of sigA and sigB recognized promoters in the BKD-operon.