TABLE 1.
The total fatty acid composition of L. monocytogenes F2365, BKD-deficient strains, and their complemented strainsd
| Strains | Percentage (wt/wt) of total fatty acids (mean ± SD) | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| SCFAs | Total SCFA | Odd-number anteiso-BCFA | Odd-number iso-BCFA | Even-number iso-BCFA | |||||||||
| C12:0 | C14:0 | C15:0 | C16:0 | Anteiso-C15:0 | Anteiso-C17:0 | Iso-C15:0 | Iso-C17:0 | Iso-C14:0 | Iso-C16:0 | Total BCFA | |||
| F2365 | 0.03 ± 0.0 | 0.61 ± 0.08 | 0.13 ± 0.01 | 11.66 ± 1.39 | 12.38 ± 1.21 | 75.05 ± 3.99 | 0.24 ± 0.07 | 5.60 ± 2.21 | 1.23 ± 0.38 | 0.01 ± 0.00 | 1.34 ± 0.50 | 83.46 ± 7.15 | |
| ∆lpd | 0.04 ± 0.0 | 5.46 ± 1.13a | 0.49 ± 0.09a | 20.14 ± 2.6a | 26.13 ± 1.37c | 56.28 ± 8.11c | 0.04 ± 0.02 | 3.87 ± 1.01 | 0.73 ± 0.02 | 0.02 ± 0.00 | 7.54 ± 1.50 | 68 ± 10.64b | |
| ∆bkdA1 | 0.09 ± 0.02 | 17.66 ± 1.9c | 4.47 ± 1.16a | 37.09 ± 0.87c | 59.32 ± 2.22c | 6.21 ± 1.44c | 0.01 ± 0.00 | 1.82 ± 0.41 | 0.78 ± 0.09 | 0.05 ± 0.01 | 24.76 ± 5.16c | 33.64 ± 7.13c | |
| ∆bkdA2 | 0.14 ± 0.0 | 10.73 ± 3.64a | 1.38 ± 0.54 | 59.27 ± 8.61c | 71.47 ± 12.88 | 2.26 ± 0.88c | 0.01 ± 0.00 | 1.68 ± 0.64 | 1.21 ± 0.00 | 0.05 ± 0.01 | 12.00 ± 5.05 | 16.81 ± 7.28c | |
| ∆bkdB | 0.04 ± 0.0 | 6.34 ± 2.28a | 1.04 ± 0.40 | 55.30 ± 5.84c | 62.70 ± 8.54b | 7.06 ± 2.90c | 0.02 ± 0.01 | 1.14 ± 0.40 | 1.03 ± 0.00 | 0.05 ± 0.01 | 16.87 ± 7.16b | 25 ± 11.07c | |
| ∆lpd_Com | 0.02 ± 0.00 | 1.45 ± 0.30 | 0.23 ± 0.06 | 12.44 ± 0.53 | 14.13 ± 0.17 | 76.33 ± 3.91 | 0.14 ± 0.03 | 2.59 ± 0.66 | 0.47 ± 0.06 | 0.01 ± 0.00 | 3.23 ± 0.83 | 82 ± 5.50 | |
| ∆bkdA1_Com | 0.01 ± 0.00 | 0.32 ± 0.05 | 0.05 ± 0.00 | 5.80 ± 0.67 | 6.17 ± 0.76 | 88.85 ± 0.05 | 0.19 ± 0.01 | 2.22 ± 0.54 | 0.33 ± 0.04 | ND | 0.97 ± 0.23 | 92.56 ± 1.41 | |
| ∆bkdA2_Com | 0.01 ± 0.00 | 0.34 ± 0.04 | 0.05 ± 0.00 | 7.09 ± 0.47 | 7.46 ± 0.54 | 86.53 ± 1.45 | 0.20 ± 0.01 | 2.74 ± 0.61 | 0.44 ± 0.04 | 0.01 ± 0.00 | 0.96 ± 0.18 | 90.88 ± 2.28 | |
| ∆bkdB_Com | 0.01 ± 0.00 | 0.33 ± 0.00 | 0.04 ± 0.00 | 6.97 ± 0.00 | 7.35 ± 0.00 | 89.28 ± 1.93 | 0.20 ± 0.02 | 2.53 ± 0.43 | 0.41 ± 0.01 | 0.01 ± 0.00 | 0.99 ± 0.14 | 93.41 ± 1.37 | |
Indicates a significant difference at P < 0.05.
Indicates a significant difference at P < 0.01.
Indicates a significant difference at P < 0.001.
The Student's t test was used to measure the statistical significance between mutants and wild-type.