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. 2024 Jun 20;206(7):e00033-24. doi: 10.1128/jb.00033-24

TABLE 1.

The total fatty acid composition of L. monocytogenes F2365, BKD-deficient strains, and their complemented strainsd

Strains Percentage (wt/wt) of total fatty acids (mean ± SD)
SCFAs Total SCFA Odd-number anteiso-BCFA Odd-number iso-BCFA Even-number iso-BCFA
C12:0 C14:0 C15:0 C16:0 Anteiso-C15:0 Anteiso-C17:0 Iso-C15:0 Iso-C17:0 Iso-C14:0 Iso-C16:0 Total BCFA
F2365 0.03 ± 0.0 0.61 ± 0.08 0.13 ± 0.01 11.66 ± 1.39 12.38 ± 1.21 75.05 ± 3.99 0.24 ± 0.07 5.60 ± 2.21 1.23 ± 0.38 0.01 ± 0.00 1.34 ± 0.50 83.46 ± 7.15
∆lpd 0.04 ± 0.0 5.46 ± 1.13a 0.49 ± 0.09a 20.14 ± 2.6a 26.13 ± 1.37c 56.28 ± 8.11c 0.04 ± 0.02 3.87 ± 1.01 0.73 ± 0.02 0.02 ± 0.00 7.54 ± 1.50 68 ± 10.64b
∆bkdA1 0.09 ± 0.02 17.66 ± 1.9c 4.47 ± 1.16a 37.09 ± 0.87c 59.32 ± 2.22c 6.21 ± 1.44c 0.01 ± 0.00 1.82 ± 0.41 0.78 ± 0.09 0.05 ± 0.01 24.76 ± 5.16c 33.64 ± 7.13c
∆bkdA2 0.14 ± 0.0 10.73 ± 3.64a 1.38 ± 0.54 59.27 ± 8.61c 71.47 ± 12.88 2.26 ± 0.88c 0.01 ± 0.00 1.68 ± 0.64 1.21 ± 0.00 0.05 ± 0.01 12.00 ± 5.05 16.81 ± 7.28c
∆bkdB 0.04 ± 0.0 6.34 ± 2.28a 1.04 ± 0.40 55.30 ± 5.84c 62.70 ± 8.54b 7.06 ± 2.90c 0.02 ± 0.01 1.14 ± 0.40 1.03 ± 0.00 0.05 ± 0.01 16.87 ± 7.16b 25 ± 11.07c
∆lpd_Com 0.02 ± 0.00 1.45 ± 0.30 0.23 ± 0.06 12.44 ± 0.53 14.13 ± 0.17 76.33 ± 3.91 0.14 ± 0.03 2.59 ± 0.66 0.47 ± 0.06 0.01 ± 0.00 3.23 ± 0.83 82 ± 5.50
∆bkdA1_Com 0.01 ± 0.00 0.32 ± 0.05 0.05 ± 0.00 5.80 ± 0.67 6.17 ± 0.76 88.85 ± 0.05 0.19 ± 0.01 2.22 ± 0.54 0.33 ± 0.04 ND 0.97 ± 0.23 92.56 ± 1.41
∆bkdA2_Com 0.01 ± 0.00 0.34 ± 0.04 0.05 ± 0.00 7.09 ± 0.47 7.46 ± 0.54 86.53 ± 1.45 0.20 ± 0.01 2.74 ± 0.61 0.44 ± 0.04 0.01 ± 0.00 0.96 ± 0.18 90.88 ± 2.28
∆bkdB_Com 0.01 ± 0.00 0.33 ± 0.00 0.04 ± 0.00 6.97 ± 0.00 7.35 ± 0.00 89.28 ± 1.93 0.20 ± 0.02 2.53 ± 0.43 0.41 ± 0.01 0.01 ± 0.00 0.99 ± 0.14 93.41 ± 1.37
a

Indicates a significant difference at P < 0.05.

b

Indicates a significant difference at P < 0.01.

c

Indicates a significant difference at P < 0.001.

d

The Student's t test was used to measure the statistical significance between mutants and wild-type.