Fig. 1.

Variation in copy numbers of AHPND-associated plasmid and toxin gene between Control and Stress groups at each time point. The relative copies of (A) the AHPND plasmid and (B) the toxin gene were determined for individuals from the Control and Stress experimental groups at each time point (n = 8 per time point T06, T12, and T24). The relative copies of the AHPND plasmid and toxin gene were normalized to the host genome copies. Statistical significance was calculated using Student’s t-test (* p < 0.05, **p ≤ 0.01)