Fig. 3.
Effect of J-domain integrity and cellular crosslinking on interactor recovery with DNAJB8. A Schematic of immunoprecipitation. B Recovery of DNAJB8 from each condition. Error bars represent standard deviation (n = 3). All differences between conditions with different DSP treatment have p < 10−5 by post hoc Tukey’s HSD after one-way ANOVA (F = 165, p = 2 × 10−7). C, D Relative association of interactors. Hsp70 chaperones and Hsp70-associated co-chaperones are shown in orange. Three biological replicates, comprising three LC–MS runs, were performed