Fig. 4.

A Proteins recovered by immunoprecipitation of ^FlagDNAJB1^WT without crosslinking (comparable to Fig. 2B) and B proteins recovered by immunoprecipitation of ^FlagDNAJB1^H32Q with crosslinking (comparable to ref. 8). Mock cells are transfected with eGFP. The experimental strategy is similar to that from Fig. 2A, except that the DNAJB1^H32Q AP-MS was preceded by cellular crosslinking with 250 µM DSP. Nine biological replicates, comprising three LC–MS runs, were performed for DNAJB1^WT, while six biological replicates, comprising two LC–MS runs, were performed for DNAJB1^H32Q. Hsp70 proteins are indicated in purple, Hsp70-associated co-chaperones are in green, and protein identified from Piette et al. [31] are italicized. The p-value cut-off corresponding to a 10% false discovery rate is indicated. The global false discovery rate for all proteins from Storey’s modification of Benjamini–Hochberg analysis is 73% for DNAJB1^WT and 5.9% for DNAJB1^H32Q