Fig. 5.
Effect of J-domain integrity and cellular crosslinking on interactor recovery with DNAJB1. This data is from distinct experiments from those reported in Fig. 4. A Recovery of DNAJB1 from each condition. Error bars represent standard deviation (n = 3). All differences between conditions have p < 0.02 by post hoc Tukey’s HSD after one-way ANOVA (F = 59, p = 1 × 10−5), except for WT + DSP and H32Q–DSP. B, C Relative association of interactors. Hsp70 chaperones and Hsp70-associated co-chaperones are shown in orange. Three biological replicates, comprising three LC–MS runs, were performed