Fig. 5. Necroptosis of PA-treated UCP1−/− NK cells could be reversed by poly I:C.
A IFN-γ, CD69, and NKG2D of UCP1−/− NK cells stimulated with poly I:C. IFN-γ production (B), LDH releasing (C), necroptosis molecules and Bcl-2 (D), mitophagy molecules (E), and p-Akt, NF-κB p65, c-Myc, HK2, and CPT1α (F), mitotracker, mitoSOX and TMRM (G) in PA-treated NKKO cells stimulated with Poly I:C. H Variations of molecules involved in mitophagy and necroptosis after splenic NK cells were treated with a cMyc siRNA. All experiments were repeated at least twice. Ns no significance; *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.