FIG. 4.

Complex formation between granzyme B and either CrmA or Serp2. Radiolabeled CrmA or Serp2 protein was synthesized by coupled transcription-translation in vitro, incubated with granzyme B, and analyzed by electrophoresis and autoradiography. (A) Denaturing (SDS) gel showing CrmA protein without granzyme B (lane 1), CrmA treated with granzyme B (lane 2), and Serp2 without (lane 3) or with (lane 4) granzyme B. The CrmA-granzyme B complex is indicated by the arrow at left, and cleaved CrmA is indicated by the arrowhead. The Serp2-granzyme B complex is marked by the asterisk on the right. (B) Time course of complex formation between granzyme B and Serp2. Serp2 protein was incubated without granzyme B (lane 1) or with granzyme B (lanes 2 to 7) for the time indicated above each lane in minutes.