TABLE 1.
Summary of pBecker1::mini-Tn5 isolates
| E. coli plasmid | Distance (kb) from Fa | Locusb | % Intactc | CPEd |
|---|---|---|---|---|
| pBecker1-1 | ∼50 | UL36 homology | ? | − |
| pBecker1-2 | ∼60 | UL39 (RR1) | 97 | ++ |
| pBecker1-3 | ∼10 | IR (IE180) | 100e | ++l |
| pBecker1-4 | ∼3 | US6 (gD) | 5 | +m |
| pBecker1-5 | ∼35 | UL28 (ICP 18.5) | 46 | − |
| pBecker1-6 | ∼35 | UL13 (kinase) | 49 | + |
| pBecker1-7 | ∼70 | UL41 (VHS) | 100f | ++ |
| pBecker1-8 | ∼30 | UL5 (helicase) | 54 | − |
| pBecker1-9 | ∼40 | no homology | ? | − |
| pBecker1-10 | <2 | US4 (gG) | [89]g | ++ |
| pBecker1-11 | ∼60 | UL22 (gH) | 100h | + |
| pBecker1-12 | ∼15 | IR (IE180) | 100i | ++l |
| pBecker1-13 | ∼30 | UL5 (helicase) | 25 | − |
| pBecker1-14 | ∼45 | UL38 (VP19c) | 7 | − |
| pBecker1-15 | ∼60 | no homology | ? | ++ |
| pBecker1-16 | ∼50 | UL21 | 70 | + |
| pBecker1-17 | ∼20 | UL19 (VP5) | 100j | + |
| pBecker1-18 | ∼70 | UL44 (gC) | 100k | ++ |
| pBecker1-19 | ∼55 | no homology | ? | − |
| pBecker1-20 | ∼35 | UL28 (ICP 18.5) | 44 | − |
| pBecker1-21 | ∼40 | UL9 (origin bp) | 25 | − |
| pBecker1-22 | ∼40 | UL29 (ICP 8) | 36 | − |
| pBecker1-23 | ∼40 | UL13 (kinase) | 90 | + |
Approximate location of the transposon insertions is listed as the distance (in kilobases) from the F plasmid in the gG ORF, based on restriction digestion analysis. The distance listed is always no more than half of the total genome size, as the genome is circular in E. coli.
Determined by sequencing the I end of the transposon.
Percentage of ORF intact 5′ of the transposon insertion site, determined by sequencing the I end of the transposon. Insertions in previously unidentified PRV loci are indicated by question marks.
Each isolate was scored for CPE following transfection into PK15 cells. Designations are as follows: no CPE resulted (−), CPE manifested with notably slower kinetics than pBecker1 (+), or CPE was similar or identical to that of pBecker1 (++).
Transposon is 41 bp upstream from the transcript start site.
Transposon is 54 bp upstream from the ORF.
The gG ORF is interrupted at the 5′ end by the F plasmid; therefore, the number given is only an indication of the transposon insertion site.
Transposon is 19 bp downstream from the ORF.
Transposon is between the E4 enhancer element and the P1 promoter of IE180.
Transposon is 2 bp downstream from the ORF.
Transposon is 118 bp downstream from ORF.
Wild-type CPE probably results from the equalization of the inverted repeats during viral replication.
CPE was restricted to small infectious foci.