Fig. 8.

Hypocotyl lengths of Col-0, myosin viii4KO, and Dex-inducible CML13,14 hpRNAi lines. Seeds were sown onto 0.5× MS agar plates with (treatment) or without (control) 5 µM Dex and cold stratified in the dark at 4 °C for 48 h. Plates were then moved to the growth chamber, still in the dark, and the hypocotyls were allowed to elongate for 5 d before imaging (A) and measuring the hypocotyl lengths with ImageJ (B). Boxes show the mean ±SD of each line on each of the control or treatment plates, whiskers portray the range of the data acquired, and each point is a single biological replicate (one-way ANOVA with Tukey’s test for multiple comparisons, with 20–45 biological replicates, *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001). White bar in (A)=1 cm, and all images are of the same magnification.