FIG. 1.

Synthesis of plus-strand RNA by using DNA templates. (A) Template d(−1/13) containing the sequence complementary to nt 1241 to 1252 of BMV RNA3 are shown with the initiation cytidylate indicated by an arrow. Changes of the 3′ ends of d(−1/13), the +2 adenylate, the +3 uridylate, and the +4 adenylate were used for RNA synthesis by BMV RdRp. The changes are indicated above the autoradiogram of the RdRp products. The positions of the 13- and 14-nt products are shown on the left. The reaction products were separated by denaturing PAGE (12% polyacrylamide) and visualized by autoradiography. (B) Summary of the effect of nucleotide changes focusing on the 3′ end of the initiation site. All results presented were from at least three independent trials. (C) Summary of the effect of nucleotide changes at positions +2, +3, and +4 in d(−1/13). (D) Effect of changes to a guanylate in the first six positions in d(−1/13) on plus-strand RNA synthesis.