FIG. 5.

The effects of nucleotide changes near the initiation cytidylate on RNA synthesis. (A) Effect of nucleotide changes near the initiation cytidylate. Changes from B2(−)46G (top sequence) are indicated in bold type. − indicates the absence of the −1 nucleotide. (B) Effects of the identity of the 3′ nontemplated nucleotide on genomic plus-strand initiation. The initiation cytidylate is indicated by an arrow. Substitutions of the 3′ nontemplated nucleotide is indicated in bold type. The synthesis directed by the different endscripts is given as a percentage relative to B2(−)46G. The results presented are from three independent trials.