FIG. 6.

Effect of multiple initiation sites on RNA synthesis. The authentic initiation site is indicated by an arrow in the first construct marked 1. Additional initiation sites added to the 3′ end of B2(−)46G are indicated by arrows 2 and 3. The three initiation sites should generate products of 46, 49, and 52 nt. RNA synthesis directed by the different initiation sites from their respective templates are presented relative to initiation from cytidylate 1 in B2(−)46G. Products initiated from the three potential initiation sites are indicated on the right as 1, 2, and 3 respectively.