Figure 5.

Senolysis, but not JAK inhibition, reduces key SASP components from senescent AECs. Four days following medium only or bleomycin treatment of AECs as previously described, medium was refreshed containing DMSO vehicle, or a dose response of Nintedanib, ABT-317, ruxolitinib, Navitoclax, or AD80 for 3 days. Supernatants were assayed for IL-6 and IL-8 using MSD kits. The number of viable cells was assessed with CellTiter-Glo reagent. Curves were fit to a four-parameter logistic function in GraphPad Prism and the IC₅₀ values are summarized in Table 1. Representative curves from 3-4 experimental repeats from 3 biological donors are shown. Error bars represent SEM of n = 2 technical replicates.