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CHF causes the loss of Nissl bodies and ultrastructural damage of neurons in the hippocampus. (A) Representative images of Nissl’s staining. (B–D) Quantifications of Nissl bodies in CA1, CA3, DG regions in the hippocampus, respectively n = 4. (E) Representative ultrastructural images of neurons shown at magnifications of ×2500, scale bars: 20 μm; ×8000, scale bars: 5 μm. Data are presented as the Mean ± SEM; *P < 0.05, and **P < 0.01 vs. Sham; n = 3.
