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Fluorescence-activated cell sorter analysis and sorting of CD4+ T cells. CD4+-enriched T cells were obtained by negative bead selection and subsequently stained for the expression of CD62L, CD45RA, and CD4. Sorting gates are shown for lymphocytes (left panel), CD4-positive T cells (middle panel), and naive and memory cells (right panel). Cell purities were always assessed immediately and 36 h postsorting and were >95%.
