FIG. 5.

HTA and accompanying HMA of amplified envelopes obtained from cDNA in plasma (P), DNA in ex vivo-sorted memory (M) and naive (N) CD4⁺ T cells, and supernatant viral culture cDNA of memory (C-M) and naive (C-N) CD4⁺ T cells in two representative patients. U, unrelated sample. For each patient, two HTA were performed, with a probe made from virus cultured from memory CD4⁺ T cells (C-M*) (left side of gel) and a probe made from virus cultured from naive CD4⁺ T cells (C-N**) (right side of gel). In patient 5 (a and b), the migration patterns between envelopes from cultured memory and naive CD4⁺ T cells differed, indicating genetic differences between viruses cultured from these two subsets; however, in patient 7 (c and d) migration occurred equally along the length of the gel, indicating the presence of viruses with genetically identical envelopes. In both patients, envelope PCR products from cultured memory CD4⁺ T cells migrated equally with those from plasma virus (left side of gel), whereas there was a retardation of migration in the plasma viruses compared to viruses from naive CD4⁺ T cells with the probe of virus cultured from naive CD4⁺ T cells (C-N**) (right side of gel), thus indicating the closer genetic similarity of viruses in plasma and memory cells than in plasma and naive cells.