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. 2024 Jul 12;6:1407409. doi: 10.3389/fmedt.2024.1407409

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© 2024 Kasim, Chillon, Eleftheriadou, Rijntjes, Minich, Zechmann and Schomburg.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterization of ghrelin-aAb signal decline with dilution. Signal strength was tested with positive serum samples in dilution experiments with assay buffer or negative serum samples. (A) Five positive serum samples (subjects A–E) were diluted with assay buffer to 50%, 25%, 12.5% and 0.1% of the original composition. The signals declined steadily with increasing dilution. (B) Five different ghrelin-aAb positive serum samples were diluted 1:1 with a negative serum sample each. Signal strength for ghrelin-aAb declined strongly, towards the arithmetic mean of both samples, indicating little to no matrix effects.