Skip to main content
. 2024 Jun 6;98(8):2487–2539. doi: 10.1007/s00204-024-03761-y

Table 10.

Characteristics of the used hepatocytes (activities by the supplier)

Pooled cryopreserved human hepatocytes (50-donor mixed gender)
Bioreclamation IVT product number X008005
Lot number HQE
Enzyme Assay Activity [jn pmol/106 cells/min]
Total P450 ECOD 79.2
UGT 7-OH-coumarin glucuronidation 426
ST 7-OH-coumarin sulfatation 35.7
CYP1A2 Phenacetin O-deethylation 41.2
CYP2A6 Coumarin 7-hydroxylation 89.9
CYP2B6 Bupropion hydroxylation 35.6
CYP2C8 Amodiaquine de-ethylation 228
CYP2C9 Tolbutamide-4′-hydroxylation 52.0
CYP2C19 S-Mephenytoin 4′-hydroxylation 14.6
CYP2D6 Dextromethorphan demethylation 20.1
CYP2E1 Chlorzoxazone-6-hydroxylation 23.9
CYP3A4 Testosterone-6β-hydroxylation 137
CYP3A4 Midazolam-1-hydroxylation 69.4
Pooled cryopreserved male Sprague Dawley rat hepatocytes
Bioreclamation IVT product number M00005
Lot number PWX
Enzyme Assay Activity [jn pmol/106 cells/min]
Total P450 ECOD 122
UGT 7-OH-coumarin glucuronidation 163
ST 7-OH-coumarin sulfatation 97.7
Cryopreserved male Syrian hamster hepatocytes
Bioreclamation IVT product number S001135
Lot number GBI
Enzyme Assay Activity [jn pmol/106 cells/min]
Total P450 ECOD 501
UGT 7-OH-coumarin glucuronidation 271
ST 7-OH-coumarin sulfatation 40.8